Clone 707 of the Friend cell, from which clone M2 was derived, also responds to treatment with dimethylsulphoxide by increased synthesis of haemoglobin. However, although the final globin mRNA content of fully induced cells is comparable, the basal level is considerably Chaussure Nike Blazer Noir higher. Moreover, there is no increase in the number of globin mRNA molecules in the nucleus as a result of induction and there is little, if any, difference in the template capacity for globin gene transcription of chromatin from induced and uninduced cells. It is postulated that clone M2 represents the original line, exhibiting linked transcriptional and post-transcriptional control of haemoglobin synthesis and that clone 707 now consists mainly of a variant in which Acheter Blazer Homme transcriptional control is relaxed.
The Nipah virus fusion (F) protein is proteolytically processed to F1 + F2 subunits. We demonstrate here that cathepsin L is involved in this important maturation event. Cathepsin inhibitors ablated cleavage of Nipah F. Proteolytic processing of Nipah F and fusion activity was dramatically reduced in cathepsin L shRNA-expressing Vero cells. Additionally, Nipah virus F-mediated fusion was inhibited in cathepsin L-deficient cells, but coexpression of cathepsin L restored fusion activity. Both purified cathepsin L and B could cleave immunopurified Nipah F protein, but only cathepsin L produced products Acheter Nike Blazer Vintage Femme of the correct size. Our results suggest that endosomal cathepsins can cleave Nipah F, but that cathepsin L specifically converts Nipah F to a mature and fusogenic form.
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